The study of modulated effects of LAMS on HL-60
- VernacularTitle:昆布多糖硫酸酯对急性髓性白血病HL-60细胞的调控
- Author:
Qing XIAO
- Publication Type:Journal Article
- Keywords:
LAMS;
HL-60 cell strain;
Modulation
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of LAMS on modulation of HL-60 cell strain.Methods:MTT was used to determine the inhibiting action in the different concentration of LAMS on HL-60 cell strain.The attitude of Sub-G1 peak in DNA histogram was examined by FCM.NF-?B expression affected by LAMS was determined by using immunohistochemical stainings.Results:With the increase of LAMS concentration,the inhibiting effects on multiplication of HL-60 cells were decreased.After treatment of HL-60 cells with 0.75?g/ml LAMS for 6h?12h?24h,the expression of P65 was the most significant at 12h,while the expression of IKb? was the most significant at 6h.The exptession of NF-?B reduced in LAMS group without time-dependent pattern.Conclusion:LAMS can induce HL-60 cells apoptosis.LAMS can modulate the expression of NF-?B.It also suggested that LAMS had an influence at molecular level.
