Seropharmacological Effect of Kangxian Ruangan Granule on Collagen-degradation Related Gene Expression in Hepatic Astrocytes
- VernacularTitle:抗纤软肝冲剂对肝星状细胞胶原降解相关基因表达的影响
- Author:
Zheng ZHOU
;
Jie CHEN
;
Chizhi ZHANG
;
Shaoxian LAO
- Publication Type:Journal Article
- Keywords:
Kangxian Ruangan Granule /pharmacology;
Hepatic astrocytes /drug effects;
Collagen /drug effects;
Matrix metalloproteinase;
Tissue inhibitor of metalloprotein ase;
Hepatic fibrosis /drug therapy(TCD);
Gene expression
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Kangxian Ruangan Granule(KRG)on the collagen degradation in hepatic astrocytes(HA)at the cellular and molecular level.Methods The medicated sera of KRG was used to i ncubate the sub-cultured HA and sodium chloride and c olchicines were taken as controls.T he content of typeⅠcollagen in the cultured su-pernatant was measured with ELISA an d then was corrected by assaying the t otal protein in cell layer.The mRNA e xpres-sions of matrix metalloproteinase -1(MMP1)and tissue inhibitor of metalloproteinase -1(TIMP -1)were analyzed by semiquantitative RT -PCR.Results The content of typeⅠcollagen in the KRG -cultured supern atant was lower than that in the other two groups(P
