Comparison of baicalin in skullcap polyploid determined by MEKC and HPLC methods
- VernacularTitle:MEKC法和HPLC法测定黄芩多倍体中黄芩苷含量的研究
- Author:
Shanlin GAO
;
Zhen LIU
;
Danni ZHU
- Publication Type:Journal Article
- Keywords:
Scutellaria baicalensis Georgi;
baicalin;
MEKC;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To determine the baicalin content in skullcap polyploid by MEKC and HPLC, and to provide the reliable method used to determine a large number of samples. Methods On the basis of methodology of MEKC and HPLC, the baicalin in Scutellaria baicalensis Georgi. was determined, the data and relevant analysis were compared. Results The data of the content determined by HPLC was similar to that by MEKC, but the former showed slightly higher 1%-3%. There was no significant variation between the two methods with higher correlation coefficient. Conclusion Both the above two methods could be accurately used to determine the baicalin in S. baicalensis. The MEKC has some advantages, such as quick, save time with lower solvent cost, and is suitable for the large number of samples in the determination of baicalin of S. baicalensis.
