Effect of nimesulide on proliferation,apoptosis and protein expression related to STAT3 signaling pathway in SGC-7901 cells
- VernacularTitle:尼美舒利对人胃癌SGC7901细胞增殖、凋亡及STAT3通路相关蛋白表达的影响研究
- Author:
Min CHEN
- Publication Type:Journal Article
- Keywords:
Signal transducer and activator of transcription 3;
Gastric cancer;
Nimesulide
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibition effect of proliferation of gastric cancer cell line SGC7901 treated with selective cyclooxgenase 2(COX-2)inhibitor,nimesulide,and the probable mechanism of cell signal transduction.Methods: SGC7901 cells were treated with nimesulide.MTT assay was used to measure the cell proliferation;Flow cytometry was used to detect cell cycle and apoptosis.The protein expression related to STAT3 signaling pathway in SGC7901 cells was examined by Western blot.Results:The growth of gastric cancer cells was obviously inhibited in a time-and dose-dependent manner after nimesulide treatment;the percentage of apoptosis was increased significantly(P
