INOCULATING COMPLETE OR C TERMINAL FRAGMENT OF A?_(42) VACCINE INDUCED NORMAL SD RATS TO PRODUCE HIGH TITER OF ANTIBODY AGAINST A?_(42)
- VernacularTitle:A?_(42)及其C端亚单位疫苗接种正常SD大鼠后产生高滴度的抗A?_(42)抗体
- Author:
Xian LIN
;
Huaqiao WANG
;
Jie XU
;
Zhibin YAO
- Publication Type:Journal Article
- Keywords:
A?;
Subunit vaccine;
Antibody;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether inoculating complete or C terminal fragment of A? 42 vaccine could induce normal rats to produce high titer of antibody against A? 42 or not. Methods The COOH\|terminals(36\|42) of beta\|Amyloid 42 were coupled with different carriers to construct subunit vaccine,which been inoculated in normal SD rats sa did the complete beta\|Amyloid 42.Antibodies against A? 42 of the serum and supernatant of homogenated brain were assessed by Western blottting and indirect ELISA.The morphology of brain,liver,spleen and kidney were observed in paraffin section by HE staining. Results After the second inoculation finished,all experiment groups began to develop antibody against A? 42 ,and the titer raising with inoculating times.After four inoculations,The titers of antibody against A? 42 in every experiment group were over than 1∶10?000.Meantime,low titer of antibody against A? 42 could also be assessed in supernatant of homogenated brain.No morphological damages could be detected in the brain,liver,spleen and kidney of the experiment animals.Conclusion\ Normal SD rats can be induced to develop high titer of antibody against A? 42 after inoculating A? 36\|42 vaccine and A? 1\|42 vaccine.There were no morphological damages in several important organs.\;[
