EFFECTS OF RECOMBINANT CILIARY NEUROTROPHIC FACTOR ON STAT3 EXPRESSION AND TYROSINE PHOSPHORYLATION IN THE INJURED NEURONS DURING PERIPHERAL NERVE REGENERATION
- VernacularTitle:周围神经再生时重组CNTF对受损神经元STAT3表达和酪氨酸磷酸化的作用
- Author:
Jiajun XU
;
Eryu CHEN
;
Changlin LU
;
Cheng HE
- Publication Type:Journal Article
- Keywords:
Peripheral nerve;
Nerve regeneration;
Ciliary neurotrophic factor;
Neuron;
Signal transducer and activator of transcription 3;
Phosphotyrosine
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects of recombinant ciliary neurotrophic factor(CNTF) on the changes of JAK\|STAT pathway and tyrosine phosphorylation in the injured neurons. Methods Sciatic nerve of rat was resected and sutured into silicone tube with local infusion of recombinant CNTF.The distribution and quantity of STAT3 and phosphotyrosine(PTyr) immunoreactivity in the neurons of L3\|L5 spinal anteriolateral nuclei and L5 spinal ganglion were observed and measured by immunohistochemical ABC method with computer image analysis. Results There was much STAT3 immunoreactivity in the neuron located in the nucleus of spinal anteriolateral nuclei.PTyr immunoreactivity showed higher in the cell membrane of spinal anteriolateral nuclei and in the cytoplasma and neucleus of spinal ganglion in CNTF group than that in SAL group.Conclusion\ The results suggest JAK\|STAT pathway in the injured motoneurons be activated and strengthened and tyrosine phosphorylation in the injured neurons be enhanced by recombinant CNTF.\;[
