Expression of Tissue Inhibitor of Metalloproteinase-2(TIMP-2) in Pichia pastoris
- VernacularTitle:TIMP-2在毕赤酵母中的克隆与表达
- Author:
Xunyou YAN
- Publication Type:Journal Article
- Keywords:
TIMP-2,Pichia pastoris,Express,Activity
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
To construct human tissue inhibitor of metalloproteinase-2 and express it in P.pastoris.The sequence was designed according to the amino acid sequence of the TIMP-2 and high-level expression sequences of P.pastoris.We gained the TIMP-2 gene by artificially synthesis method.The gene was cloned into pPIC9 to construct a recombinant vector pPIC9-T2.The vector was transformed into P.pastoris by electroporation,through phenotype selection and induced with methanol we get the expression cell.Separation,purification and bioactivity analysis of the expressed products was performed.
