Effects of substance P on the activity of intestinal mucosal mast cells in rats with multiple organ failure
- VernacularTitle:P物质对多器官功能衰竭时肠黏膜肥大细胞活性的调节
- Author:
Cheng LAN
;
Chengwei TANG
- Publication Type:Journal Article
- Keywords:
Intestinal mucosal mast cells;
Multiple organ failure;
Substance P;
Histamine;
Tumor necrosis factor-?
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Multiple organ failure (MOF) has been regarded as a continuous, uncontrolled inflammatory response. Intestinal mucosal mast cells (IMMC) may be involved in MOF. Substance P (SP), one of gut peptides, is an important regulator in the neuro-endocrine-immune network. However, the effects of SP on IMMC, especially in the case of MOF, remain unclear. This study was aimed to investigate the effects of SP on IMMC in the development of MOF. Methods The rat model of MOF was established by injecting zymosan. After thirty minutes of the injection, SP was given through tail veins at the dose of 20 nmol/kg weight and 0.2 nmol/kg weight. The concentrations of histamine and tumor necrosis facfor-?(TNF-?) in plasma and intestine tissues were measured. The pathological alterations of essential organs including intestine, liver, kidney and lung were examined under light microscope. Their corresponding functions were reflected with ALT, Cr and PO 2. The ultrastructure of the IMMC was also observed under a transmission electronic microscope. Results Compared with the controlled rats, those injected with SP showed much more serious inflammatory response under light microscope. Both ALT and Cr increased by about 50%, but PO 2 decreased by about 40%. Histamine level in the intestinal tissue of the rats treated with SP remarkably decreased, whereas the plasma histamine level did not show any significant changes. The level of TNF-? was higher in the intestinal tissue of the rats treated with SP but no change in plasma, and the degranulation of IMMC under transmission electronic microscope was more obvious.Conclusions SP may trigger MOF through acting on IMMC which may release inflammatory mediators such as histamine and TNF-?.
