Effect of taurine on proliferation and apoptosis of rat hepatic stellate cells in vitro
- VernacularTitle:牛磺酸对离体肝星状细胞增殖及凋亡的影响
- Author:
Yuexiang CHEN
;
Xingrong ZHANG
;
Weifen XIE
- Publication Type:Journal Article
- Keywords:
Taurine;
Hepatic stellate cell;
Proliferation;
Apoptosis
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of taurine on proliferation / apoptosis of rat hepatic stellate cells in culture and the possible mechanism involved. Methods Rat hepatic stellate cells were incubated with different concentration of taurine. Cell proliferation was assessed by MTT colorimetric assay, cell cycle and apoptosis were analysed by flow cytometry, apoptotic morphology was examined by vital staining of acridine orange, c-jun and c-fos expression was determined by immunocytochemistry and computer video text analysis system. Results Administration of 5-50 mmol/L taurine into culture medium had no toxicity to hepatic stellate cells, but could significantly inhibit hepatic stellate cell proliferation in dose-dependent manner, increase the number of cell in G 0/G 1 phase and decrease the numbers in S phase. Taurine could also markedly inhibit c-jun and c-fos expressions ( P
