Study on mutations of K-ras gene in pancreatic diseases
- VernacularTitle:K-ras基因在胰腺疾病中突变的检测及其临床意义
- Author:
Yuexin REN
;
Guoming XU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
Chronic pancreatitis;
K-ras oncogene;
Mutation
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of K-ras mutations in pancreatic diseases, specifically in the diagnosis of pancreatic carcinoma. Methods One hundred and seventeen surgical-resected pancreatic specimens, including 24 pancreatic ductal adenocarcinoma, 19 peritumoral ductal atypical hyperplasia, 58 peritumoral ductal hyperplasia, 19 normal duct at the tumor-free margin and 24 ductal lesion from chronic pancreatitis were obtained, and DNA was extracted from the specimens. Codon 12 K-ras mutations were examined using two-step polymerase chain reaction (PCR) combined with restriction enzyme digestion, non radioisotopic single-strand conformation polymorphism (SSCP) analysis and automated DNA sequencing. Results K-ras mutation rate in the lesion of pancreatic carcinoma was 79.2%(19/24), which was significantly higher than 33.3%(8/24) in the lesion of chronic pancreatitis( P
