Screening potentially drug resistant and multidrug resistant genes in NPC cells with metastatic ability
- VernacularTitle:具有转移能力鼻咽癌细胞株候选抗药与多药耐药相关基因的筛选
- Author:
Weiyi FANG
;
Zhen LIU
;
Siming XIE
;
Qingping JIANG
;
Shuang WANG
;
Xin LI
;
Kaitai YAO
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal neoplasms;
Oligonucleotide array sequence analysis;
Neoplasm metastasis;
Drug resistance,multiple
- From:
Cancer Research and Clinic
2008;20(7):436-438,448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen potential genes associated with drug resistance and multidrug resistance. Methods Microarray with 8000 genes was used to detect the different expression of 5-8F cells and 6-10B cells. Subsquently, genes of drug resistance and multidrug resistance were screened by MILANO online programme. Semiquantitative RT-PCR was utilized to confirmed the reliability of differentially expressed genes. Results 283 genes were identified the differential expression. Of these, 85 genes were shown to be upregulated and 98 downregulated. After the analysis of MILANO, 4 genes including UGT1A9 (15.85 folds),MVP(6.77 folds), CAV1(2.49 folds) and HIF1A(2.67 folds) with higher expression in 5-8F cells were found to be likely associated with drug resistance and multidrug resistance. Subsquently, semiquantitative RT-PCR confirmed the reliability of differential expression of these 4 genes. Conclusion Differentially expressed genes shown in NPC 5-8F cells compared to 6-10B cells with the identification of online MILANO program analysis are likely associated with drug resistance and mnltidrug resistance of NPC cells with the ability of metastasis.
