Determination of ginsenoside Re、Rg1 in Yianhuisheng Oral Liquor by HPLC
- VernacularTitle:HPLC测定益安回生口服液中人参皂苷Re、Rg1的含量
- Author:
Cha LIU
;
Minghao ZHANG
;
Yu ZHONG
- Publication Type:Journal Article
- Keywords:
Yianhuisheng Oral Liquor;
ginsenoside Re、Rg1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a HPLC method for the determination of ginsenoside Re、Rg1 in Yianhuisheng Oral Liquor(Radix Ginseng Rubra, Radix Aconiti Lateralis Preparata, Scorpio etc.) Methods: RP-HPLC was used to quantitative analysis. The Synchropak C 4 column (250mm?4.6mm, 5?m) was used, and mobile phase was composed of acetonitrile-0.05% H 3PO 4(18∶82). Detection wavelength was at 203nm, the flow rate was 1.0 mL?min -1 , the injection volume of sample was 10?L and of reference 8?L. Results: The linear response range from 0.432 ～3.456?g of Re(r=0.9992, n=5), 0.360～2.88?g of Rg1(r=0.9998, n=5), respectively. The recovery of Re: 97.0%, RSD=1.4%, the recovery of Rg1: 96.8%, RSD:=1.1%, respectively. Conclusion: The method was proved to be simple, rapid, accurate and reproducible, and suitable for content determination of the oral liquor.
