Determination of cannabidiol in hemp seed oil by HPLC
- VernacularTitle:HPLC法测定火麻仁油中大麻二酚的含量
- Author:
Gang ZHANG
;
Jiangning GUO
;
Kaishun BI
- Publication Type:Journal Article
- Keywords:
hemp seed oil;
cannabidiol;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop the analysis method to determine the content of cannabidiol in the hemp seed oil by HPLC. Methods The chromatographic condition was Irregular-H-C 18 column (250 mm? 4.6 mm, 10 ?m). A mixture of methanol-acetonitrile-water-acetic acid (25∶50∶25∶0.4) was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was 220 nm at room temperature. Results The calibration curve for cannabidiol showed good linear correlation within the concentration range of 1.2 — 9.6 ?g/mL (r=0.999 4). The average recovery and RSD was 94.6% and 1.9% (n=9) respectively. Conclusion The method is convenient, reliable and with good reappearance.
