Content Determination of Paeonol in Liuwei Dihuang Pill by SPE - HPLC
- VernacularTitle:SPE-HPLC法测定六味地黄丸中丹皮酚的含量
- Author:
Weiling LI
;
Shengwen LIN
;
Yuzhu HU
- Publication Type:Journal Article
- Keywords:
@ Liuwei Dihuang Pill /chemistry;
Paeonol/analysis;
@Solid - phase extraction;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop SPE - HPLC method for the assay of paeonol in Liuwei Dihuang Pill (LDP). Methods Separation was performed on a Hypersil ODS(2) column (250 mm?4. 6 mm, 5?m) . The mobile phase consist of methanol - 2 % acetic acid (55 :45, v/v) . The flow rate was 1 mL/min. The UV detection was set at 275 nm. Results Paeonol had a good linear relation in the range of 0. 03998 ~ 0. 7996 ug, the average recovery of honeyed bolus of LDP was 102. 9 % and the RSD was 1.2%; the average recovery of concentrated bolus of LDP was 101. 5 % and the RSD was 1.8%. Conclusion The method is simple, sensitive and rapid and it can be used to determine the paeonol content of LDP.
