Effects of Adrenomedullin1-50 on vascular cell adhesion molecule-1 expression in isolated rat heart
- VernacularTitle:肾上腺髓质素心肌保护作用机制的实验研究
- Author:
Yuting BAI
;
Cun LONG
;
Zhongfan TU
- Publication Type:Journal Article
- Keywords:
Myocardial reperfusion injury ?Myocardial ischemic ?Vascular cell adhesion molecules-1 ? Adrenomedullin
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of adrenomedullin1-50 (Adm1-50) on vascular cell adhesion molecule-1 (VCAM-1) expression in isolated rat heart. Methods: Twenty-four male Sprague-Dawley rat, weighting 300 to 350g, were randomly divided into A, B, C, D group (n=6 for each group). The isolated rat hearts were perfused in a Langendorff mode for 20 min and followed by 60 min of global ischemia. Then, all groups were reperfused with Kerbs-Henseleit bicarbonate for 60 min, but group B, C, D were perfused with buffer in the presence of Adm1-50 10-9mol/L, 10-8mol/L, and 10-7mol/L respectively for 15 min after the onset of reperfusion. The post-ischemic change of creatine kinase-isoenzyme (CK-MB) in coronary effluent and the expression of myocardial VCAM-1 mRNA were measured. Results: After I/R, Adm1-50 dose-dependently decreased the expression of VCAM-1 and the CK-MB activity, The ratio of VCAM-1/GAPDH mRNA were 1 20?0 52, 1 10?0 45, 0 60?0 31, 0 50?0 36 for group A, B, C, D, respectively (P
