EFFECTS OF ANAESTHESIA ON THE HEAT SHOCK RESPONSE OF MICE
- VernacularTitle:麻醉对小鼠热休克反应的影响
- Author:
Cunshuan XU
;
Shuang ZHOU
;
Sanqiang LI
- Publication Type:Journal Article
- Keywords:
Heat shock response;
Heat shock protein 70;
proteinases;
Anaestesia;
Sober;
Mouse
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to understand and evaluate the significance and action of the whole body's regulation in the organism's heat shock response, this paper analyzed the difference between the anaesthetic and sober mice in the same heat shock condition. Methods The anaesthetic and sober mice were treated respectively in 40, 42,44 and 46℃ for 30?min and then recovered for 24?h in normal raise. The heat shock protein 70(HSP70) in mouse livers were checked by Western blot.The acid and neutral proteinase activities in mouse livers were checked by renatured electrophoresis of proteinases. Results HSP70 synthsis in the sober mouse livers was decreased by 44\|46℃, but it was stronger in the anaesthetic mouse livers. The acid proteinase activities in the sober and anaesthetic mouse livers basically indicated a positive relationship with HSP70 synthesis, but the neutral proteinase activities showed a negative relationship.Conclusion Based on the results, it was inferred that the anaesthetic mouse livers had a higher heat\|resistance than that of the sober mouse livers, and that the stress response of the mouse livers was regulated by two levels of cells and whole body, which also involved in other physiological and biochemical actions except the heat shock protein synthesis. [
