Expression of decorin and its mRNA in skin lesion of scleroderma mice
10.3760/cma.j.issn.1007-7480.2010.07.012
- VernacularTitle:核心蛋白多糖及其mRNA在硬皮病模型小鼠皮损中的表达
- Author:
Haitao LI
;
Rongya YANG
;
Xin FAN
;
Wenling WANG
;
He ZHU
;
Congmin WANG
- Publication Type:Journal Article
- Keywords:
RNA,messenger;
Scleroderma,localized;
Gene expression;
Decorin
- From:
Chinese Journal of Rheumatology
2010;14(7):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of decorin and its mRNA in the skin of normal mice and lesion of seleroderma mice to explore the possible role of decorin in the pathogenesis of scleroderma. Methods Scleroderma mice model induced by Bleomycin were developed and the expression of decorin in the scleroderma mice and normal control mice were determined by immunohistochemistry and RT-PCR. Comparisons between groups were performed with rank test and t test. Results According to the comparison of the histopathology of skin and lung and the skin collagen content between the model group and the control group, the construction of scleroderma model was successful. The results showed that the expression of decorin had no significant change in each group examined by immunohistochemistry, and the expression of decorin had high expression in normal control mice (0.60±0.15) and low expression in scleroderma mice (0.26±0.03) by RT-PCR. Conclusion The low expression of decorin is detected in scleroderma mice. It is suggested that this low expression maybe related to the high expression of TGF-β1 and TGF-β2 in scleroderma mice to neutralize or consume decorin.
