The study of bladder cancer cell line irradiated with high intensity focused ultrasound
- VernacularTitle:高强度聚焦超声破坏膀胱癌细胞株的实验研究
- Author:
Chuan LIU
- Publication Type:Journal Article
- Keywords:
High intensity focused ultrasound;
Bladder tumor;
Cell line;
Ultrasound therapy
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility and mechanism of high intensity focused ultrasound (HIFU) in the treatment of bladder cancer. Methods:After irradiating the bladder cancer cell line T24,the number of survival cells was detected with MTT col-orimetry. The relationship between HIFU dosage and cell survival rate was analysed. The activity of alkaline phosphatase (ALP) and creatine- kinase (CK) in culture medium was determined and the structures of the cells were studied. Results: The relationship between sound intensity and cell survival rate is in power series. With increasing sound intensity ,cell survival rate decreased sharply. The relationship between irradiation time and cell survival rate is linear. When sound intensity was 2300 W/cm2, the optimum irradiation time was 7 seconds. After irradiation ,cell growth was suppressed and cell numbers reduced continuously until 6 hours later. This indicated that there was necrobiosis in these cells. With the prolongation of the irradiation, the activity of ALP、CK in culture medium increased and reached it's peak at two seconds. Under optical and electronic microscopes, the damage to the cancer cell was irreversible. Conclusion :HIFU is an effective ,minimally invasive treatment option for bladder tumor.
