Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats
- VernacularTitle:幼年鼠内毒素性脑水肿模型及脑组织钙离子和钙调素表达的研究
- Author:
Mei LI
- Publication Type:Journal Article
- Keywords:
Endotoxin;
Brain edema;
Calcium;
Calmodulin
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To establish a simple, reproducible brain edema model induced by endotoxin in infant rats and explore the role of intracellular free calcium and calmodulin (CaM). in the development of brain edema. Methods: A total of 45 infant rats were divided randomly into endotoxin treated( n = 36) and control( n = 9) group. The animals were intraperitoneally injected with 10mg/kg of endotoxin or saline respectively. The brain tissue intracelluar free [Ca2 + ] as well as the expression of CaM was detected by fluorescence and protein hybridizaton methods respectively. The tissue water content,Evans Blue content and pathology under electronic microscope were also observed. Results:Brain water and EB content were remarkably increased in endotoxin treated animals compared with those in the control, in which the intracellular free [Ca2+ ] was also significantly increased and the expression of CaM up - regulated. Electron microscopic studies revealed that the blood - brain barrier(BBB)was disrupted with neuronal degeneration,neurogliocyte swelling and necrosis. Conculsion :These results suggest that LPS increases the intracellular free [Ca2+ ] ,and activates the CaM - dependent function via Ca2+ /CaM pathway,which might relate to the increased BBB permeability and brain edema formation.
