Determination of the contents of aristolochic acid A in Zhushalian capsule by HPLC
- VernacularTitle:HPLC测定朱砂莲胶囊中马兜铃酸A的含量
- Author:
Yong CHEN
- Publication Type:Journal Article
- Keywords:
Zhushalian capsules;
Aristolochic acid A;
HPLC
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the contents of aristolochic acid A in Zhushalian capsules. Methods : Column hypersil C18 (250mm ? 4.6mm,5?m). Mobile phase:Solution of methanol - water - glacial acetic acid (79 : 29 : 1). Detection wavelength: 315nm. Flow rate: 1.0ml/min. Results: Aristolochic acid A was completely separated and no interference was found. The recovery rate was 98. 34 % . RSD was 0.04 % ( n = 5 ) . Conclusion : This method is simple, sensitive, accurate and reliable.
