Determination of Psoralen and Isops oralen Contents in Fukangbao Capsul e by HPLC
- VernacularTitle:HPLC法测定复康宝胶囊中补骨脂素与异补骨脂素的含量
- Author:
Jingai TIAN
;
Qingpeng DU
;
Weixin WANG
;
Ruicha LIN
- Publication Type:Journal Article
- Keywords:
Fukangbao Capsules /chemictry;
Psoralen /analysis;
Chromatography,high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a reversed -phase HPLC m ethod for the determination of psora len and isopsoralen in Fukangbao capsule.Methods The contents of Psoralen and Isopsoralen were assayed on a ODS -C 18 column with a mobile phase of methanol -water(40∶60)at a column temperature of 35℃.The fl ow rate was 1.0mL /min.The detection wave-length was at 247nm.Results The linear ranges of Psoralen and Iso psoralen were 1.05～10.52?g /mL(r =0.9999)and1.02～10.20?g /mL(r =0.9999)respectively.Their recoveries were within 97.5%(Psoralen)and 100.8%(Isopsoralen),and both of their RSD were 0.6%.Conclusion This method is simple,rapid and accu rate and suitable for quantity -limiting control of Fukan gbao capsule.
