Quantitative Determination of Emod in and Chrysophanic Acid in Compound Rhubarb Spray by RP -HPLC
- VernacularTitle:RP-HPLC法测定大黄复方喷雾剂大黄素、大黄酚的含量
- Author:
Xinchun WANG
;
Shixiang HOU
;
Changming YANG
;
Zhilin HU
;
Yon YUAN
- Publication Type:Journal Article
- Keywords:
Compound Rhubarb spray /chemistry;
Emodin /analysis;
Chrysophanic acid /analysis;
Chromatogra-phy,high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determi nation of emodin and chrysophanic acid in Compound Rhubarb Spray by HPLC.Methods The ODS column was applied.methanol∶water(87∶13)served as mobile phase.The detectio n wavelength was at 254nm and the flow r ate was 0.65ml /min.Results The average recovery of emodin was 99.48%with relative standard deviation being 1.75%(n=9)and the average recovery of chrysoph anic acid was 101.46%with rela-tive standard deviation being 2.85%(n=9).Conclusion The method was simple,rapid and reli able.It is suitable for the quality control of Compound Rhubarb Spray.
