Determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid by HPLC
- VernacularTitle:HPLC法测定银黄口服液中绿原酸和黄芩苷的含量
- Author:
Jiang QIAN
- Publication Type:Journal Article
- Keywords:
Yinhuang Oral Liquid;
chlorogenic acid;
baicalin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae). Methods: LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL?min -1 . The detection wavelength was set as 318nm. Results: For chlorogenic acid, the linear range was 0.2568 ～ 2.0544 ?g, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ～7.9168?g, and the average recovery rate was 98.0% with RSD =0.9%. Conclusion: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.
