The prevalence rate of three species of integrons in multi-drug resistant Pseudomonas aeruginosa and investigation of class Ⅰ integron structure in Jiangyin area
- VernacularTitle:江阴地区三类整合子在多重耐药铜绿假单胞菌中的流行率及Ⅰ类整合子结构研究
- Author:
Feng LIU
;
Zhongming LU
;
Jianbo ZHOU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Integrons;
Drug resistance,Multiple
- From:
International Journal of Laboratory Medicine
2008;29(4):305-306,310
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistance rate of Pseudomonas aeruginosa to several commonly used antibiotics,as well as the prevalence status of three species of integrons in multi-drug resistant Pseudomonas aeruginosa from 2005 to 2006 in our hospital,and to identify the genetic structure of class Ⅰ integron in in our hospital.Methods Drug resistance slip method was applied to detecting the resistant rates of 100 isolates of Pseudomonas aeruginosa to ciprofloxacin,cefotaxime,gentamicin and imipenem.Boiling method was adopted to abstract DNA template of 100 isolates of Pseudomonas aeruginosa,and PCR assay adopted to identify the prevalence rate of three species of integrons and class Ⅰ integron structure.Results The resistant rates of 100 isolates of Pseudomonas aeruginosa to ciprofloxacin,cefotaxime,gentamicin,cefoxitin,tobramycin and imipenem were 32%,45%,82%,37%,39% and 43% respectively.The prevalence rates of three species of integrons was as follows:class Ⅰ 53%,Ⅱ 21% and Ⅲ 5%.Sequence analysis displayed there existed box gene dfrA17 and aadA2 in variable region of class Ⅰ integron.Conclusion The resistant rates of Pseudomonas aeruginosa to four drugs are elevated,especially to gentamycin.Multi-drug resistance may be partly due to prevalence of class Ⅰ integron.
