Study on regulatory effects of interleukin-6 on placental growth factor expression in trophoblast cells
- VernacularTitle:白细胞介素6在滋养层细胞胎盘生长因子表达调控中的作用
- Author:
Wen PENG
;
Sen JIANG
;
Minsheng DAI
- Publication Type:Journal Article
- Keywords:
Trophoblast;
Pregnancy proteins;
Interleukin-6
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression pattern of Placental growth factor (PlGF) and regulatory effects of interleukin-6 (IL-6)on PlGF expression in the primary cultured trophoblast cells. Methods Cytotrophoblast cells werecollected by trypsin-collagenase digestion and Percoll gradient centrifugation for primary culture. Reverse transcriptase polymerase chain reaction(RT-PCR) were resorted to demonstrate the expression pattern of PlGF mRNA in trophoblast cells cultured in vitro. The effects of IL-6 with different concentration(100,10,1 and 0.1 ?g/L) and duration (6,12,24 and 48 h) on PlGF expression were observed. Results The 185 bp and 248 bp bands of PlGF were showed by RT-PCR. PlGF expression correlated with IL-6. PlGF began to increase at 6 h, and reached the climax at 12 h when recultured with 100 ?g/L IL-6. Conclusions PlGF expression has time and dose dependance on IL-6. It may play an important role in early pregnancy.
