Studies on transplantation of bone marrow stromal cells transferred by angiogenin gene in ischemic myocardium
- VernacularTitle:携带Ad.ANG的骨髓基质细胞移植于缺血心肌的实验研究
- Author:
Zhigang LI
;
Ju MEI
;
Baoren ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardial Ischemia Bone Marrow Cells Angiogenesis,physiologic, Adenovirus Transplantation
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the protective effect of bone marrow stromal cells (BMSCs) transferred by Ad?ANG ex vivo on ischemic myocardium. Methods: ELISA method was used to assay the expression and secretion of angiogenin (ANG) after Ad?ANG transfection of BMSCs ex vivo. Then BMSCs with Ad?ANG were transplanted into ischemic myocardium of isogenic Lewis rats. 4 weeks later, the parameters of heart function, such as EF and EDLV, were examined by echocardiography. Survival and differentiation of transplanted BMSCs and angiogenesis were appraised by histology and transmission electron micrography. Results: ANG was found in both lysate and culture medium after transfection of BMSCs. A maximum expression of ANG was observed at 4-7 days after transfection and could still be assayed 15 days later. 4 weeks later after transplantation in the BMSCs with Ad?ANG group, heart function improved better than the single BMSCs group(P
