THE REGULATORY MECHANISM OF SUBSTANCE P ON THE TENSION OF HUMAN UMBILICAL VEIN AND THE CALCIUM ION OF ENDOTHELIAL CELLS
- VernacularTitle:P物质对人脐静脉张力及内皮细胞Ca~(2+)的调控及其机制
- Author:
Zhongxiang YAO
;
Wenqin CAI
- Publication Type:Journal Article
- Keywords:
Calcium ion;
Endothelial cell;
Patch\|clamp technique;
Substance P;
Confocal laser scanning microscopy;
Human umbilical vein
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the regulatory mechanism of substance P on the tension of human umbilical vein and the calcium ion of endothelial cells. Methods The tension was recorded by conventional physiological recording methods,and confocal laser scanning microscopy and patch\|clamp technique were used to observe the concentration of intracytosolic free calcium ion and the opening probability of membrane calcium ion channel of cultured human umbilical vein endothelial cells. Results Substance P induced the endothelial\|dependent relaxing of human umbilical vein and the increasing of the concentration of intracytosolic free calcium ion and of the opening probability of calcium ion channel in the cultured human umbilical vein endothelial cells.Conclusion\ Substance P could be function as the activator of the releasing of the intracellular stored calcium ion,and the inflowing of calcium ion from the outside cell bodies to relax human umbilical vein.
