EFFECT OF CO-CULTURED VASCULAR SMOOTH MUSCLE CELLS ON PDGF-B mRNA EXPRESSION OF ENDOTHELIAL CELLS UNDER SHEAR STRESS
- VernacularTitle:切应力作用下联合培养的血管平滑肌细胞对内皮细胞PDGF-BmRNA表达的影响
- Author:
Yuquan LI
;
Dong WANG
;
Yan ZHANG
;
Xingzhong CONG
;
Zonglai JIANG
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cells;
Shear stress;
Platelet\|derived growth factor;
Endothelial cells
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The effect of vascular smooth muscle cells (VSMCs) on the PDFG\|B mRNA expression of Co\|cultured endothelial cells (ECs) under shear stress had been studied in order to provide some experimental data for preventing the development of restenosis resulted from VSMCs proliferation in the vascular graft. Methods The level of PDGF\|B mRNA of ECs cultured alone and Co\|cultured with VSMCs exposed to shear stress were investigated by the methods of in situ hybridization and image analysis. Both controls of ECs cultured alone and Co\|cultured with VSMCs were maintained under static conditions. Results In static condition, the content of PDGF\|B mRNA expression of Co\|cultured ECs was lowered in comparison with ECs alone\|cultured. Under shear stress, PDGF\|B mRNA expression of Co\|cultured ECs was increased in transient manner at 1hour after the onset of shear stress and then returned to the level lower than the static level of Co\|cultured ECs at 6hours after being exposed to shear stress. The transient increasing time of PDGF\|B mRNA expression of Co\|cultured ECs reached a maximum earlier than that of ECs cultured alone.Conclusion Under shear stress, PDGF\|B mRNA expression of ECs Co\|cultured with VSMCs is lowered, which may be favor of inhibiting VSMCs proliferation.
