Genetic polymorphisms in glutathione S-transferases T1, M1 and P1 and susceptibility to reflux esophagitis
- VernacularTitle:谷胱甘肽转硫酶T1、M1和P1基因多态与反流性食管炎易感性
- Author:
Minli WANG
;
Jiali JIANG
;
Tianyou WANG
- Publication Type:Journal Article
- Keywords:
Glutathione S-transferase;
Genetic polymorphisms;
Reflux esophagitis
- From:
Chinese Journal of Digestion
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between genetic polymorphism in glutathione S-transferases T1, M1 and P1 and susceptibility to reflux esophagitis. Methods Genomic DNA was isolated from peripheral blood from the patients with reflux esophagitis (n=109), cases with negative endoscopy reflux disease (NERD) (n=97) and controls (n=97). Genes GSTM1 and GSTT1 were detected by multiplex PCR. A A→G polymorphism of codon 104 of the gene GSTP1 was detected using PCR-based restriction fragment length polymorphisms ( PCR-RFLP ) with Alw26I. Results The frequency of variant GSTP1 genotypes (*A/*B and*B/*B) was found to be 40.4% among the patients with reflux esophagitis compared with 24.7% among cases with gastroesophageal reflux without esophagitis and 21.6% among controls (P
