Hepatic stellate cells may exist a local renin-angiotensin-aldosterone system
- VernacularTitle:肝星形细胞存在局部肾素-血管紧张素-醛固酮系统
- Author:
Jing ZHANG
;
Chunhua ZONG
;
Dingguo LI
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cell;
Renin-angiotensin-aldosterone system;
Liver fibrosis
- From:
Chinese Journal of Digestion
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study was to elucidate whether there was a local renin-angiotensin-aldosterone system (RAAS)in rat hepatic stellate cells(HSC)and the immortalized rat HSC line—HSC-T6. Methods HSC was isolated by perfusion of collagenase and pronase to liver. The renin activity, angiotensin Ⅱ(Ang Ⅱ) and aldosterone(ALD) concentration in the cells were assayed by radio-immunoassay. Angiotensin converting enzyme(ACE) activity was detected by ultra-violet spectrophotography. The expression of angiotensin Ⅱ type 1 receptor(AT1R) was determined by immunohistochemistry. RT-PCR method was used to assay the mRNA expression of renin, angiotensinogen, ACE, AT1R and CYPⅡB2(the critical enzyme of aldosterone synthesis). Results Ang Ⅱ, ALD, renin and ACE activity could be detected in HSC and HSC-T6. Immunohistochemistry showed that both of them could express AT1R. mRNAs of renin, ACE, AT1R and CYPⅡB2 were also detected in both of them. Conclusions Both rat HSC and the immortalized rat HSC line HSC-T6 possessa a local RAAS.
