Expressions of tumor related gene NAG6, NAG-7 and BRD7 in gastric cancers
- VernacularTitle:肿瘤相关基因NAG6、NAG-7、BRD7在胃癌中的表达
- Author:
Xiaomei ZHANG
;
Shourong SHENG
;
Xiaoyan WANG
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
Genes;
Gene expression
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective NAG6, NAG 7 and BRD7 genes were novel tumor related genes identified recently. This study was designed to examine the expression of these genes in gastric cancer and matched paracancerous tissues and to investigate their roles in the occurrence and development of gastric carcinoma. Methods The expression levels of NAG6, NAG 7 and BRD7 in 34 cases of human gastric cancer and matched paracancerous tissues were analyzed by RT PCR, Northern blot analysis and Dot hybridization. Results The expression of NAG6 was absent in 61.8% gastric cancer tissues(21/34). The expression of NAG6 was significantly down regulated in gastric cancer tissues compared with that in paracancerous tissues ( P 0.05 ). The expression rates of NAG 7 in gastric cancer and paracancerous tissues were 88.2% (30/34) and 82.3% (28/34) respectively. BRD7 was expressed in all gastric cancers and matched paracancerous tissues. The results of RT PCR, Northern blot analysis and Dot hybridization all showed that the expressions of NAG 7 and BRD7 did not display any difference between gastric cancer and matched paracancerous tissues. Meanwhile, Dot hybridization also confirmed that NAG6 was significantly down expressed in gastric cancer. Conclusions NAG6 gene was significantly down regulated in gastric cancer tissues. The down regulation of this gene may play a key role in occurrence and development of gastric cancer, however it may be not associated with lymph node or distance metastasis. The expression levels of NAG 7 and BRD7 were not altered in gastric cancer. It seems that NAG 7 and BRD7 gene may not contribute to the occurrence and development of gastric carcinoma.
