Determination of Eugenol in Dikou Lizhong Wan by HPLC Combined with Solid-Phase Extraction
- VernacularTitle:丁蔻理中丸中丁香酚含量的固相萃取-高效液相色谱测定法
- Author:
Jinxiong YU
;
Binghui ZHU
- Publication Type:Journal Article
- Keywords:
Dikou Lizhong Wan/chemistry;
eugenol/analysis;
@solid-phase extraction;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of eugenol in Dikou Lizhong Wan. Methods: The sample was first purified on Sep-Pak C18 microcolumn. The chromatographic conditions were as follow: Kromasil C18 chromatographic column (200mm′4.6mm , i.d. 5mm), methanol-water mixed solution (60 ∶40) as mobile phase , the detection wavelength at 270 nm and the flow rate being 1.0 mL?min-1. Results: The average recovery of eugenol was 99.0 %and RSD= 1.8 %(N=6). Conclusion: This method was simple, convenient, rapid,accurate and reliable and it can be used to control the quality of Dikou Lizhong Wan.
