Isolation,purification and identification of polysaccharide of Cistanche deserticola
- VernacularTitle:肉苁蓉多糖的分离、纯化和鉴定
- Author:
Qunli ZENG
;
Yanan HUO
;
Junhao MAO
;
Yifa ZHENG
- Publication Type:Journal Article
- Keywords:
polysaccharide of Cistanche deserticola Y. C. Ma (CDPS);
column chromatography;
spectrum analysis
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Object The polysaccharide (CDPS) was isolated, purified and identified from Cistanche deserticola Y. C. Ma, a Chinese materia medica. Methods The polysaccharide was extracted with hot water and precipitated by alcohol. Protein in the precipitates was removed by Sevag method. The products were further purified with column chromatography on DEAE-Sephadex A-50 and Sephacryl S-200. The CDPS was idendified by IR spectrum and UV (200-400 nm) scanning spectrum. Results IR spectrum indicates that there are typical characteristic absorption peaks of polysaccharides. UV scanning spectrum shows that there are no absorption peaks of protein and nucleic acid at point 280 nm and 260 nm. Conclusion The CDPS was identified as homogeneous one.
