EXPRESSION OF SONIC HEDGEHOG′S FUNCTIONAL RECEPTOR-PATCHED IN CULTURED NEURAL STEM CELL
- VernacularTitle:音猬因子的功能受体斑片在培养神经干细胞中的表达
- Author:
Hanpeng XU
;
Peizhen HU
;
Lin GOU
;
Ping ZHANG
;
Gong JU
- Publication Type:Journal Article
- Keywords:
Sonic hedgehog;
Patched;
Receptor;
Cell culture;
Neural stem cell;
RT\|PCR;
in situ hybridization
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether cultured neural stem cell expresses morphogen molecule sonic hedgehog′s functional receptor patched. Methods Cultured neural stem cell clones were subjected to RT\|PCR after passaged several times in vitro ,the amplification production was sequenced and labeled by digoxingemin and in situ hybridization technique was carried out to detect the cryosection of the neural stem cell clones. Results Most cells in the stem cell clones were positive for sonic hedgehog functional receptor patched,no significant difference was found among the positive cells and the center and peripheral of the stem cell clones.Conclusion\ The sonic hedgehog signal transduction may have important role in the proliferation and differentiation process of neural stem cell.\;[
