INCREASING THE SINGLE-CLONE FORMED RATE OF NEURAL STEM CELLS FROM ADULT RATS
- VernacularTitle:提高成年大鼠神经干细胞单克隆形成率的方法
- Author:
Zhen HUANG
;
Guohua JIN
;
Xinhua ZHANG
;
Meiling TIAN
;
Jianbing QIN
;
Huijun XU
- Publication Type:Journal Article
- Keywords:
Neural stem cell;
Clone;
Differentiation;
Adult rats
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Modified the medium that can increase single\|clone formed rate and confirmed the single clone spheres had the multipotential of differentation. Methods We modified the medium, that is, the medium contained half of primary culture medium and half of fresh culture medium. A great deal of neurospheres dervied from a single cell were plated averagely into 24 well plates and added into the DMEM differentiation medium (containing serum). After culturing for 14 days, cultures were stained with the neuronal\| ang glial\|specific markers (MAP\|2 for neurons, GFAP for astrocytes and CNP for oligodendrocytes). Results Each 96 well plate containing half of primary culture medium generated two to three single clone spheres, in control plate containing only fresh medium generated half to one single clone sphere. After differentiation, these cell clones expressed MAP\|2, GFAP and CNP positive respectively.Conclusion\ Using half of primary culture medium can increase single\|clone formed rate and these cell clones had the multipotential of differentiation.\;[
