The influence of protein-tyrosine phosphatase inhibitor on the vacuolating activity of VacA
- VernacularTitle:蛋白酪氨酸磷酸酶抑制剂对VacA空泡活性的影响
- Author:
Yiqi DU
;
Guoming XU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Helicobactor pylori;
Protein tyrosine phosphatase inhibitor;
VacA
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been reported that VacA receptor of H pylori on gastric cell membrane is belong to the receptors of protein tyrosine phosphatase(RPTP) family, which are associated with signal transduction of cells. However the mechanism of vacuolation is still unknown. There may be a new way to reduce the toxicity of H.pylori by interfering the signal transduction. Methods The concentrates of supernatants from H.pylori liquid culture was used as rough VacA. Protein tyrosine phosphatase inhibitor (PTPI) and protein tyrosine kinase inhibit or(PTKI) which were diluted and combined with rough VacA were incubated with SGC 7901 cells for 24 hours. Results PTPI could inhibit the vacuolating activity with a minimal concentration of 2.7 ?mol/L( A 550 =0.46?0.06 vs 0.59?0.04, P
