The effects of indomethacin on the expression of CDK_2, CDK_4, Bcl-2, Bax and p21~(WAF1/CIP1) protein in the human colon adenocarcinoma cell line
- VernacularTitle:吲哚美辛对结肠癌细胞CDK_2、CDK_4、p21~(WAF1/CIP1)、Bcl-2及Bax蛋白表达的影响
- Author:
Meihua XU
;
Guiying ZHANG
;
Zhaoxia XIE
- Publication Type:Journal Article
- Keywords:
Indomethacin;
HCT116 cell line;
Proliferation;
Apoptosis;
Cyclin dependent kinase
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been known that NSAIDs can inhibit the growth of tumors through cyclooxygenase, but it is unknown that whether there are other mechanisms involved. It is essential to detect the effect of indomethacin for proliferation and apoptosis in the HCT116 and explore its anti tumor mechanism. Methods Human colon adenocarcinoma cell line HCT116 was treated with different concentration of indomethacin for 24 h and CDK 2,CDK 4, Bcl 2, Bax and p21 WAF1/CIP1 protein were detected by Western blot. Results Indomethacin down regulated the expression of CDK 2, CDK 4, Bcl 2 and up regulated the expression of p21 WAF1/CIP1 , however, the expression of Bax remained unchanged. Conclusions Inhibiting proliferation and inducing apoptosis contribute to the anti tumor activity of indomethacin by down regulating the expression of CDK 2, CDK 4, Bcl 2 and up regulating the expression of p21 WAF1/CIP1 .
