Study of activation of human T cells with superantigen SEA
10.3969/j.issn.1000-8861.2001.02.002
- VernacularTitle:超抗原SEA对外周血T细胞的活化作用
- Author:
Guilian XU
;
Xihua ZHU
;
Jin YANG
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):85-87,104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the activation of human T cells with superantigen SEA. Methods T cells proliferation, IL-2 production, DNA synthesis, cell phenotype and apoptosis induced by the SEA in the first or restimulation were detected. Results It was shown that 100 ng/mL was the optimal activation concentration, IL-2 production arrived at top level in the third day, SEA activated CD4+ and CD8+T with the same degree, T cells start apoptosis in response to SEA restimulation within 24 hours and apoptosis disappeared through addition of rhIL-2. Conclusions There were correlation between activation and SEA concentration or stimulation period； SEA activated both CD4+ and CD8+T cells without change the cell phenotype.
