Determination of ChrysophanoL in Kuiyanqing Xiangkouye by HPLC
- VernacularTitle:HPLC法测定溃炎清香口液中大黄酚的含量
- Author:
Junxuan YANG
;
Li CHEN
;
Xiaodong PU
- Publication Type:Journal Article
- Keywords:
Kuiyanqing Xiangkouye / chemistry;
Chrysophanol / analysis;
Chromatography , high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a determination method of chrysophanol in Kuiyanqing Xiangkouye . Methods:HPLC with DiamondTM C18 column(4.6?200mm,5?m)was used . Methanol: 0.1 %phosphoric acid(85:15)was used as mobile phase . The flow rate was 1.0mL/min . The detection wavelength was at 254nm . Results:The linearity of chrysophanol was in the range of 0.06462～1.03392 ?g . The recovery was 97.67 %and RSD was 1.37 %. Conclusion:This method is convenient and with a good resolution and can be used for the quality control of Kuiyanqing Xiangkouye .
