Determination of Icarin in Naoling Granule by HPLC
- VernacularTitle:HPLC法测定脑灵颗粒剂中淫羊藿苷的含量
- Author:
Daishui XIONG
;
Songling FAN
;
Xujiang YUAN
;
Xiuping LUO
- Publication Type:Journal Article
- Keywords:
Naoling Granule/chemistry;
@Icarin /analysis;
Chromatography , high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determinating icarin content in Naoling Granule. Methods: Water-soluble ethyl-acetate-extracting polyamide column purification was adopted. The content of icarin was determined by HPLC. C18 column was used, mobile phase was methanol:0.1mol/mL sodium phosphate dibasic (6:5) and detection wavelength was at 270nm. Results: Icarin has a good linearity in the range of 0.24～1.2?g ( r=0.9998). The average recovery was 98.23 %and RSD was 1.2 %(N=5). Conclusion: The method was simple with good reproducibility and can be used for the content determination of icarin in Naoling Granule.
