SCREENING OF THE DIFFERENTIALLY EXPRESSED GENES IN LEFT OPTIC TECTA OF PIGEONS WITH THEIR LEFT-RETINA REMOVED
- VernacularTitle:单眼视网膜摘除鸽视盖差异表达基因的筛选
- Author:
Lixia LV
;
Lei XU
;
Cuixiang YANG
;
Xueli LI
;
Yao WANG
- Publication Type:Journal Article
- Keywords:
Suppression subtractive hybridization;
Expressed sequence tag;
Optic tecta;
Pigeon
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the differentially expressed genes in optic tecta of pigeon with monooccular deprivation. Methods Using suppression subtractive hybridization technique,the differentially expressed cDNA library for the left optic tecta of pigeons was constructed;recombinant clones were sequenced randomly and further proved to be the true positive by reverse Northern blot analysis. Results 150 of EST (expressed sequence tags) were sequenced and 16 of them proved true positive,which included those homologous to known genes and those having no matches.138 of ESTs were assigned Accession Number by Genbank.Conclusion\ Our data provided to be the useful clues for studying the development of visual system and the molecular construction of vision center system.
