EFFECTS OF ATP ON U937 CELL APOPTOSIS
- VernacularTitle:ATP对人白血病细胞U937凋亡的影响
- Author:
Guizhi LV
;
Ying HU
;
Guiguo ZHANG
;
Zhongxiang LIN
- Publication Type:Journal Article
- Keywords:
ATP;
U937 cells;
Apoptosis;
Cx43;
F\|actin;
Vinculin
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of U937 cell (human histocytic lymphema) apoptosis induced by ATP. Methods ATP (0\^23?g/L) was added into U937 cell cultured medium as experiment group, no ATP adding group as the control. Using immunofluorescent cytochemistry method to demonstrat the expresses of Cx43, F\|actin, vinculin. Results ATP induced U937 cell to form apoptotic bodies. Expresses of Cx43, F\|actin, vinculin were increased in ATP treated U937 cells.Conclusion ATP could induce U937 cell apoptosis. Apoptotic bodies formed from ATP treated U937 cells was correlated closely with express of Cx43, F\|actin, vinculin, It indicats that Cx43, F\|actin, vinculin play important roles in U937 cell apoptosis induced by ATP.
