The expressional changes of mitogen-activated protein kinase signaling transduction associated genes and their significance in gastric cancer cells induced by parvovirus H-1 infection
- VernacularTitle:细小病毒H-1感染对有丝分裂原激活蛋白激酶信号传导相关基因表达的影响
- Author:
Zhihua RAN
;
Jiong LIU
;
Ying FENG
- Publication Type:Journal Article
- Keywords:
Gastric cancer cells;
Parvovirus H-1;
Mitogen-activated protein kinase;
Signal transduction
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antineoplastic parvovirus H-1 induced cell death signaling pathways and their possible mechanisms in gastric carcinoma cells. Methods By using RT-PCR method, the mRNA expressional changes of mitogen-activated protein kinase (MAPK) signaling transduction pathway related genes were measured in gastric adenocarcinoma HGC27 cells infected by H-1 virus. Results The RT-PCR amplification results displayed that 48 h after HGC-27 cells were infected by H-1 virus, the expression of CREB was increased, the expression of ERK1, STAT2, p38-?, MEK2, ?-RAF and MTK1 were remarkably decreased, however, the expression of JNK2, ETS and ERK2 had no apparently change. Conclusions The cytotoxic effects of parvovirus H-1 might affect the gene expression involved in MAPK signal transduction pathway in gastric carcinoma HGC27 cells. It indicates that H-1 virus might interfere with specific cellular signal transduction pathways of gastric carcinoma cell to induce cell death. In conclusion, it was considered that modified and reconstructed parvovirus H-1 would be a very valuable tool in the anti-tumor study.
