Improved RP-HPLC method for determination of ferulic acid concentration in human serum
- VernacularTitle:RP-HPLC测定人血清中阿魏酸浓度的改进方法
- Author:
Jingqun HUANG
;
Xi HUANG
;
Li ZHANG
;
Rui XU
;
Ping REN
- Publication Type:Journal Article
- Keywords:
ferulic acid;
serum;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop an improved RP-HPLC method for determining ferulic acid in the human serum. Methods The determination was carried on RP-HPLC, using Kromasil-C 18column, methanol-water-acetic acid (36.4∶63∶0.6) as mobile phase with a flow rate of 1.0 mL/min and at the detection wavelength of 322 nm, and p-hydroxybenzaldehyde was used as internal standard. The serum sample was deproteinized with acetonitrile to extract ferulic acid. Results The calibration curve showed good linearity over the range of 9.94-159.04 ng/mL (r=0.992 5). RSD was less than 10% within day and day-to-day, the average recovery was 99.77% and the minimal concentration in serum was 5 ng/mL. Conclusion This method, which is simple, rapid, sensitive, reproducible and low toxic, is appropriate for the quantitative determination of ferulic acid in the human serum.
