Determination of scutellarin in Erigeron breviscapus and its extract by capillary electrophoresis
- VernacularTitle:毛细管电泳法测定灯盏花及提取物中灯盏花乙素含量
- Author:
Yi RAO
;
Huizhen WEI
;
Yiming WANG
;
Guoa LUO
- Publication Type:Journal Article
- Keywords:
capillary electrophoresis;
Erigeron breviscapus (Vant.) Hand.-Mazz.;
dengzhanhuasu;
scutellarin
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a method for the determination of scutellarin in Erigeron breviscapus (Vant.) Hand. -Mazz. and its extract (dengzhanhuasu) by capillary electrophoresis. Methods Separation was carried out in an uncoated fused silica capillary 57 cm ?50 ?m (ID). Meanwhile, a running voltage 20 kV, 40 mmol/L borax (pH 8.50 with H 3PO 4) buffer and a UV detector at 280 nm were adopted. Results The calibration curve showed good linearity over the range of 0.1-4.0 mg/mL (r=0.998 5). The average recovery was higher than 98.0%. Conclusion The method is proved to be simple, rapid and accurate, and can be used for quality control of E. breviscapus and its extract (dengzhanhuasu).
