Rapid induction of mRNAs for liver regeneration genes by hepatopoitin and partial hepatectomy
- VernacularTitle:肝细胞生成素及肝部分切除快速诱导肝再生相关基因Tec的表达
- Author:
Ge WANG
;
Enren LENG
;
Lu HU
- Publication Type:Journal Article
- Keywords:
Hepatopiotein;
Partial hepatectomy;
Liver regeneration;
Immediate early gene
- From:
Chinese Journal of Digestion
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective After partial hepatectomy, hepatopoietin(HPO) increase rapidly. The present study was designed to investigate how rhHPO and partial hepatectomy induce the expression of immediate early gene. Methods The Wister rats were divided into hepatectomy group and control group. The different gene expression within 1 h after 2/3 partial hepatectomy and in primary cultured hepatocytes system were examined by using representational difference analysis. Results In the expressed sequence tag(EST) library, most of these genes were identified as immediate early genes, and one new gene Tec that might be associated with liver regeneration in the EST library was found. Moreover, the Tec gene was rapidly induced by 2/3 partial hepatectomy. The expression peak achieved within 1 2 h after operation. HPO can rapidly induce the expression of these genes(c fos, LRF 1 and Tec, etc.) in primarily cultured rat hepatocytes. Conclusion rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. This is the first report that Tec gene is an immediate early gene related to liver regeneration.
