Assay of Danshensu and Protocatechuic Aldehyde in Compound Danshen Dropping Pill by HPLC
- VernacularTitle:高效液相色谱法测定复方丹参滴丸中丹参素和原儿茶醛的含量
- Author:
Xin HONG
;
Suiqing MI
;
Ningsheng WANG
- Publication Type:Journal Article
- Keywords:
Compound Danshen dropping pill / chemistry;
@Danshensu /analysis;
@Protocatechuic Aldehyde /analysis;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To separate and determine two constituents of Danshensu and protocatechuic aldehyde in compound Danshen Dropping Pill. Methods: The chromatographic conditions were as follow: Nucleosil C18 column, a mixture of methanol-water-acetic acid glacial (19∶80∶1) as the mobile phase with a flow rate of 1 mL per minute and the detection wavelength at 279 nm. P-hydroxybenoic acid was used as internal standard. Results: The calibration curves were linear in the range of 10～80 mg/L (r=0.9995) for Danshensu and 2～16 mg/L (r=0.9996) for protocatechuic aldehyde . The average recovery of Danshensu and protocatechuic aldehyde were 99.4 %and 99.5 %respectively. Conclusion: This method is accurate, simple and convenient, rapid and reproducible and can be used for the quality control of compound Danshen Pill.
