Quantitative determination of Ophiopogonin D in Zhe Ophiopogon japonicus(Thunb.)Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. by HPLC-ELSD
- VernacularTitle:ELSD-HPLC法测定浙麦冬、川麦冬中麦冬皂苷D含量的方法研究
- Author:
Jianpin YU
;
Jianfeng SHAO
;
Ming ZHU
- Publication Type:Journal Article
- Keywords:
Ophiopogon japonicus /chemistry;
@Ophiopogon D /analysis;
TCD place of production;
@HPLC-ELSD
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
HPLC-ELSD was used to determine the content of Ophiopogonin D in Zhe Opiopogon japonicus(Thunb.) Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. The chromatographic conditions were as follow: Alltima C18 column (250mm?4.6mm) and the column temperature at 30℃, drift tube temperature at 98 ℃with Gas flow rate being 2.8 L/min and acetonitrile : water (55∶45) as mobile phase with the flow rate being 1.0 mL/min. There was a good linearity (r=0.9992) within the range of 0.2565～6.4125 ?g. The average recovery rate was 102.0%and RSD=2.1%.
