EXPRESSION OF GDNF,GFAP AND NGFRp75 BY OLFACTORY ENSHEATHING CELLS IN ADULT RAT AND GOLDEN HAMSTER
- VernacularTitle:GDNF、GFAP和NGFRp75在成年大鼠和金黄地鼠嗅球成鞘细胞的免疫组织化学研究
- Author:
Lei YANG
;
Changman ZHOU
;
Hongyan MAI
;
Enhua YU
- Publication Type:Journal Article
- Keywords:
Olfactory ensheathing cell;
Glia derived neurotrophic factor(GDNF);
Immunochemistry;
Rat;
Golden hamster
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of GDNF in the olfactory ensheathing glia cells of the adult rats and golden hamster for studying the mechanisms of the OECs in the CNS regeneration. Methods The immunohistochemical staining was performed on the slides of adult olfactory bulb from 3 rats and 3 golden hamsters.According to the primary antibody,tissue slides were divided into 3 groups:rabbit polyclonal GDNF,the rabbit polyclonal GFAP and rabbit polyclonal NGFRp75(Santa Cruz Biotechnology)respectively,and followed by the rabbit ABC immunoreactive staining system. Results It revealed that GDNF was expressed by cells in the first two lines of olfactory bulb from rat and golden hamster.The GFAP and NGFRp75 were also expressed in the same position from other two groups of slides respectively.Conclusion The olfactory ensheathing cell could secret GDNF in the progress of mediating neuronal survival and axonal elongation.
