Multi-glycosidorum triptery suppresses production of interleukin-4 by splenocytes in oxazolone-induced murine colitis
- VernacularTitle:雷公藤多甙对噁唑酮诱导小鼠肠炎模型脾淋巴细胞因子的作用及其机制
- Author:
Ying HAN
;
Gang CHEN
;
Yingxin SONG
- Publication Type:Journal Article
- Keywords:
Inflammatory bowel disease;
Oxazolone;
Multi glycosidorum triptery;
Interferon ?;
Interleukin 4
- From:
Chinese Journal of Digestion
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro effect of multi glycosidorum triptery (MGT) on cytokine production by splenocytes of oxazolone (OXZ) induced colitis in murine model. Methods Six mg of OXZ (in 50% of ethanol) was administered in male SJL/J mice intrarectally to induce colitis and mice were sacrificed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0.1 mg/ml or 0.01 mg/ml was added to the culture medium of splenocytes. Production of IFN ? and interleukin 4 (IL 4) in the supernatant was measured by ELISA. Results The production of IFN ? was suppressed by both 0.01 mg/ml and 0.1 mg/ml of MGT [normal control ( 1.24 ? 0.13 ) pg/ml→(0.97?0.26) pg/ml→(0.87?0.18) pg/ml, P
